GelRed Nucleic Acid Stain 10,000X in water - 0.5 ml
High-sensitivity nucleic acid stain for gel electrophoresis, providing clear, sharp bands, supplied by Gentaur's warehouse.
GelRed™ Staining Protocol (Precast Method)
Product: GelRed™ Nucleic Acid Stain, 10,000X in water
Volume: 0.5 ml (sufficient for 100+ gels depending on usage)
Applications: dsDNA, ssDNA, RNA visualization
I. Materials Required
Agarose
TAE or TBE buffer
DNA samples + loading dye
GelRed™ 10,000X in water
Gel casting tray and combs
Microwave or hot plate
Electrophoresis apparatus
UV or Blue LED transilluminator
🧫 II. Precasting Gel with GelRed™
This method avoids post-staining and reduces handling time.
1. Prepare Agarose Gel:
Dissolve agarose in 1X TAE or TBE buffer (e.g., 1 g agarose in 100 mL buffer for 1% gel).
Heat until completely melted (microwave or hot plate).
2. Cool Gel:
Let the gel solution cool to about 60°C (touch warm, not hot).
3. Add GelRed™:
Add 5 µL of 10,000X GelRed™ to 50 mL of molten agarose.
Mix gently (avoid bubbles).
✅ Dilution Factor: 1:10,000
Do not add GelRed™ to very hot agarose — may degrade stain.
4. Cast the Gel:
Pour into tray, insert combs, and allow to solidify (~30 minutes).
5. Run the Gel:
Load samples and run electrophoresis as usual.
6. Visualize DNA:
Use UV or blue light transilluminator (blue preferred to reduce DNA damage).
Use orange filter if imaging with blue light.
🧪 III. Post-Staining Method (if desired)
If not precast, you can post-stain:
1. Dilute GelRed™:
Add 5 µL of 10,000X GelRed™ to 50 mL water or buffer.
2. Stain Gel:
Submerge gel in staining solution.
Incubate 30 minutes with gentle agitation (light-protected).
🔐 Storage & Safety
Store at room temp (RT) or 4°C, protected from light.
Non-mutagenic, safer than ethidium bromide (see Biotium's safety data).
Dispose of according to institutional biosafety guidelines.