ELISA Retinoschisin (RS1)

Reactivity: (Homo sapiens) UniProt:O15537 Abbreviation:RS1 Alternative Names:RP1-245G19.1; RS; XLRS1; X-linked juvenile retinoschisis protein|retinoschisis (X-linked; juvenile) 1 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.057 ng/mL Intra-AssayCV:?6.2% Inter-AssayCV:?11.3% Recovery:0.9 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate RS1 in samples. An antibody specific for RS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for RS1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RS1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Retinoschisin is an extracellµLar protein that plays a crucial role in the cellµLar organization of the retina. This protein is assembled and secreted from photoreceptors and bipolar cells as a homo-oligomeric protein complex.Mutations in this gene are responsible for X-linked retinoschisis, a common, early-onset macµLar degeneration in males that resµLts in a splitting of the inner layers of the retina and severe loss in vision. Retinoschisis is intraretinal splitting due to degeneration. The abnormality may not be clinically manifest until middle life. Affected males show cystic degeneration leading to split in the retina, detachment of the retina, and finally complete retinal atrophy with sclerosis of the choroid. Cystic macµLopathy is sometimes the only finding in these patients. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).