ELISA Ribonuclease, RNase A family 2 (RNASE2)

Reactivity: (Homo sapiens) UniProt:P10153 Abbreviation:RNASE2 Alternative Names:EDN; RNS2; Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.128 ng/mL Intra-AssayCV:?4.3% Inter-AssayCV:?7.8% Recovery:0.89 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate RNASE2 in samples. An antibody specific for RNASE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRNASE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for RNASE2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNASE2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Eosinophil-derived neurotoxin is a distinct cationic protein of the eosinophil's large specific granµLe known primarily for its ability to induce ataxia, paralysis, and central nervous system cellµLar degeneration in experimental animals (Gordon phenomenon). Rosenberg et al. (1989) isolated a 725-bp cDNA clone for EDN. The open reading frame encodes a 134-amino acid polypeptide with a molecµLar mass of 15.5 kD and a 27-residue N-terminal hydrophobic leader sequence. The sequence of the mature polypeptide was identical to that reported for urinary ribonuclease and to the N-terminal sequence of liver ribonuclease. Similarities to the ribonucleases of pancreas and angiogenin indicate that EDN belongs to the ribonuclease mµLtigene family. This gene is also symbolized RNS2 for ribonuclease 2. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).