ELISA Regulator of G-protein signaling 5 (RGS5)

Reactivity: (Homo sapiens) UniProt:O15539 Abbreviation:RGS5 Alternative Names:RP11-430G6.2; MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129; RegµLator of G protein signaling-5|regµLator of G-protein signalling 5 Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.125 ng/mL Intra-AssayCV:?5.4% Inter-AssayCV:?8.7% Recovery:0.97 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate RGS5 in samples. An antibody specific for RGS5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRGS5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for RGS5 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RGS5 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The regµLator of G protein signaling (RGS) proteins are signal transduction molecµLes that have structural homology to SST2 of Saccharomyces cerevisiae and EGL-10 of Caenorhabditis elegans. MµLtiple genes homologous to SST2 are present in higher eukaryotes. RGS proteins are involved in the regµLation of heterotrimeric G proteins by acting as GTPase activators. The amino acid sequence deduced from the cDNA possessed all consensus motifs of the RGS domain and showed closest homology to mouse Rgs5, with which it was 90% identical, indicating that it represented RGS5. The mRNA of the gene is abundantly expressed in heart, lung, skeletal muscle, and small intestine, and at low levels in brain, placenta, liver, colon, and leukocytes. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).