ELISA Regulator of G-protein signaling 19 (RGS19)

Reactivity: (Homo sapiens) UniProt:P49795 Abbreviation:RGS19 Alternative Names:GAIP; RGSGAIP; G alpha interacting protein|G protein signalling regµLator 19|guanine nucleotide binding protein alpha inhibiting activity polypeptide 3 interacting protein|regµLator of G-protein sig Application:ELISA Range:78.1-5000 pg/mL Sensitivity:27 pg/mL Intra-AssayCV:?5.3% Inter-AssayCV:?8.4% Recovery:0.97 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate RGS19 in samples. An antibody specific for RGS19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRGS19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for RGS19 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RGS19 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Sequence analysis predicted that GAIP is a 24.6-kD, hydrophilic protein that lacks a transmembrane domain and contains mµLtiple potential phosphorylation sites. GAIP shares approximately 31% amino acid identity with regµLator of G protein signaling-1 (RGS1) and -2 (RGS2); the 125-amino acid core domains of GAIP, RGS1, and RGS2 share approximately 64% homology. Northern blot analysis detected a 1.6-kb GAIP transcript in lung, placenta, liver, heart, and pancreas, with almost no expression detected in brain, skeletal muscle, and kidney. Yeast 2-hybrid binding analyses showed that throµgh its core domain, GAIP interacts strongly with GNAI3 and weakly with GNAI2, but does not interact with GNA11. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).