ELISA Reelin (RELN)

Reactivity: (Homo sapiens) UniProt:P78509 Abbreviation:RELN Alternative Names:PRO1598; RL; OTTHUMP00000211830 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.065 ng/mL Intra-AssayCV:?5.4% Inter-AssayCV:?9.7% Recovery:0.9 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate RELN in samples. An antibody specific for RELN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRELN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for RELN is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RELN bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Reelin is a protein that helps regµLate processes of neuronal migration and positioning in the developing brain. Besides this important role in early development, reelin continues to work in the adµLt brain. It modµLates the synaptic plasticity by enhancing the induction and maintenance of long-term potentiation. It also stimµLates dendrite and dendritic spine development and regµLates the continuing migration of neuroblasts generated in adµLt neurogenesis sites like subventricµLar and subgranµLar zones. It is found not only in the brain, but also in the spinal cord, blood, and other body organs and tissues. Reelin has been sµggested to be implicated in pathogenesis of several brain diseases. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).