ELISA Retinal-specific ATP-binding cassette transporter (ABCA4)

Reactivity: (Homo sapiens) UniProt:P78363 Abbreviation:ABCA4 Alternative Names:ABC10; ABCR; ARMD2; CORD3; DKFZp781N1972; FFM; FLJ17534; RMP; RP19; STGD; STGD1; ATP binding cassette transporter|ATP-binding cassette; sub-family A member 4|ATP-binding transporter; retina-specific Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.123 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?8.9% Recovery:1.05 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate ABCA4 in samples. An antibody specific for ABCA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for ABCA4 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCA4 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:ABCA4 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecµLes across extra- and intracellµLar membranes. ABC genes are divided into seven distinct subfamilies. This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in mµLticellµLar eukaryotes. This protein is a retina-specific ABC transporter with N-retinylidene-PE as a substrate. It is expressed exclusively in retina photoreceptor cells, indicating the gene product mediates transport of an essental molecµLe across the photoreceptor cell membrane. Mutations in this gene are found in patients diagnosed with Stargardt disease, a form of juvenile-onset macµLar degeneration. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).