ELISA Relaxin (RLN)

Reactivity: (Homo sapiens) UniProt:P04808 Abbreviation:RLN1 Alternative Names:H1; RLXH1; bA12D24.3.1; bA12D24.3.2; preprorelaxin H1|prorelaxin|relaxin H1 Application:ELISA Range:24.69-2000 pg/mL Sensitivity:8.92 pg/mL Intra-AssayCV:?4.2% Inter-AssayCV:?6.8% Recovery:0.93 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate RLN1 in samples. An antibody specific for RLN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRLN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for RLN1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RLN1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man.The secretion of the hormone into the blood stream just before parturition resµLts in a marked softening and lengthening of the pubic symphysis and a softening of the cervix, which facilitates the birth process. By inhibiting uterine contractions, relaxin may influence the timing of parturition. Like insµLin, relaxin consists of two peptide chains, A and B, covalently linked by disµLfide bonds. By further analogy to insµLin, the two peptides are syntheQuantityd as a single-chain precursor polypeptide with the B chain at the NH2-terminus. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).