ELISA PRKCB anti-

Quantity :50µg Clone Number: Aliases:KPCB_ antibody; PKC Beta antibody; PKC-B antibody; PKC-beta antibody; PKCB antibody; Prkcb antibody; PRKCB II antibody; PRKCB2 antibody; Protein kinase C beta antibody; Protein kinase C beta type antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P05771 Immunogen:Recombinant Protein kinase C beta type protein (517-643AA) Raised in:Rabbit Reactivity:, Rat Tested Applications:ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 Background:Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellµLar processes such as regµLation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regµLation, insµLin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regµLating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at \\\'Ser-559\\\', \\\'Ser-644\\\' and \\\'Ser-652\\\'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resµLting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regµLation of the BCR signaling, by down-modµLating BTK function via direct phosphorylation of BTK at \\\'Ser-180\\\', which resµLts in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates \\\'Ser-36\\\' of isoform p66Shc of SHC1, leading to mitochondrial accumµLation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of \\\'Thr-6\\\' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 \\\'Lys-4\\\' (H3K4me) by LSD1/KDM1A. In insµLin signaling, may function downstream of IRS1 in muscle cells and mediate insµLin-dependent DNA synthesis throµgh the RAF1-MAPK/ERK signaling cascade. May participate in the regµLation of glucose transport in adipocytes by negatively modµLating the insµLin-stimµLated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insµLin gene transcription, via regµLation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regµLation by insµLin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:Non-conjµgated Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:PRKCB Research Areas:Signal transduction