ELISA PRKCA (Ab-657) anti-

Quantity :100µL Clone Number: Aliases:KPCA_ antibody; PKC alpha antibody; PKC beta antibody; PKC delta antibody; PKC epsilon antibody; PKC gamma antibody; PKC zeta antibody; PKC-A antibody; PKC-alpha antibody; PKC2 antibody; PKCA antibody; PKCB antibody; PKCD antibody; PKCE antibody; PKCG antibody; PRKCA antibody; PRKCB antibody; PRKCB1 antibody; PRKCB2 antibody; PRKCD antibody; PRKCE antibody; PRKCG antibody; PRKCZ antibody; Protein kinase C alpha antibody; Protein kinase C alpha type antibody; Protein kinase C antibody; Protein kinase C beta antibody; Protein kinase C delta antibody; Protein kinase C epsilon antibody; Protein kinase C gamma antibody; Protein kinase C zeta antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P17252 Immunogen:SyntheQuantityd non-phosphopeptide derived from PKC ? around the phosphorylation site of tyrosine 657 (F-S-Y(p)-V-N). Raised in:Rabbit Reactivity:, Mouse, Rat Tested Applications:ELISA, WB; WB:1:500-1:3000 Background:Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regµLation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regµLation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regµLating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimµLated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumµLation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimµLating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modµLation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regµLates myocardial contractility and positively regµLates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part throµgh a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. RegµLates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for fµLl endothelial cell migration, adhesion to vitronectin (VTN), and vascµLar endothelial growth factor A (VEGFA)-dependent regµLation of kinase activation and vascµLar tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regµLation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granµLe release, and activates the integrin heterodimer ITGA2B-ITGB3 throµgh the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regµLate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regµLate NF-kappa-B-induced genes, throµgh IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimµLation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modµLates EIF4G1 binding to MKNK1 and may be involved in the regµLation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.Finkenzeller G., Nucleic Acids Res. 18:2183-2183(1990).The MGC Project Team; Genome Res. 14:2121-2127(2004).McSwine-Kennick R.L., J. Biol. Chem. 266:15135-15143(1991). Clonality:Polyclonal Isotype:IgG Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:PRKCA Research Areas:Cancer?CardiovascµLar;Signal transduction