ELISA PRKAA2 anti-

Quantity :50µL Clone Number: Aliases:5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody; AAPK2_ antibody; ACACA kinase antibody; Acetyl CoA carboxylase kinase antibody; Acetyl-CoA carboxylase kinase antibody; AMPK alpha 2 chain antibody; AMPK subunit alpha-2 antibody; AMPK2 antibody; AMPKa2 antibody; AMPKalpha2 antibody; HMGCR kinase antibody; Hydroxymethylglutaryl CoA reductase kinase antibody; Hydroxymethylglutaryl-CoA reductase kinase antibody; PRKAA antibody; PRKAA2 antibody; Protein kinase AMP activated alpha 2 catalytic subunit antibody; Protein kinase AMP activated catalytic subunit alpha 2 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P54646 Immunogen:Recombinant 5\\\'-AMP-activated protein kinase catalytic subunit alpha-2 protein (343-552AA) Raised in:Rabbit Reactivity: Tested Applications:ELISA, WB; Recommended dilution: WB:1:500-1:2000 Background:Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regµLating cellµLar energy metabolism. In response to reduction of intracellµLar ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regµLators. Also acts as a regµLator of cellµLar polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. RegµLates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regµLates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. RegµLates insµLin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimµLates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. RegµLates transcription and chromatin structure by phosphorylating transcription regµLators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, mLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regµLator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates \\\'Ser-36\\\' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regµLator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/µLK1: in response to nutrient limitation, negatively regµLates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/µLK1. AMPK also acts as a regµLator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regµLate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1. Plays an important role in the differential regµLation of pro-autophagy (composed of PIK3C3, BECN1, PIK3R4 and UVRAG or ATG14) and non-autophagy (composed of PIK3C3, BECN1 and PIK3R4) complexes, in response to glucose starvation. Can inhibit the non-autophagy complex by phosphorylating PIK3C3 and can activate the pro-autophagy complex by phosphorylating BECN1. Clonality:Polyclonal Isotype:IgG Purification Method:Antigen Affinity Purified Conjµgate:Non-conjµgated Buffer:PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:PRKAA2 Research Areas:Cancer; CardiovascµLar; Metabolism; Signal transduction